| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD163 was used as the immunogen for the CD163 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CD163 Antibody is a anti-CD163 Rabbit antibody Recombinant Rabbit Monoclonal clone 32C14 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human.
Key elements and design rationale
- Target: CD163
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32C14, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Cluster of Differentiation 163 is a type I transmembrane glycoprotein belonging to the scavenger receptor cysteine rich superfamily. Research using CD163 antibody has shown that it binds hemoglobin-haptoglobin complexes with high affinity, promoting their clearance from circulation. This function prevents oxidative damage and inflammation caused by free hemoglobin. By regulating hemoglobin metabolism, CD163 protects tissues from injury following hemolysis.
Studies with CD163 antibody have revealed additional roles in immune regulation. CD163 functions as an anti-inflammatory receptor by triggering secretion of anti-inflammatory cytokines such as IL-10 upon ligand binding. Shedding of CD163 from the cell surface produces soluble CD163, which circulates in plasma and serves as a biomarker of macrophage activation and inflammation. Elevated soluble CD163 levels are observed in conditions such as sepsis, liver disease, and autoimmune disorders.
CD163 expression is tightly regulated by cytokines and environmental signals. Research using CD163 antibody has demonstrated that IL-6 and glucocorticoids induce expression, while pro-inflammatory stimuli such as lipopolysaccharide downregulate it. This dynamic regulation reflects the role of CD163 in resolving inflammation and restoring homeostasis.
In pathology, CD163 has emerged as a biomarker of tumor-associated macrophages. Studies with CD163 antibody have shown that high CD163 expression correlates with immunosuppressive macrophage populations in cancers, supporting tumor progression and poor prognosis. In cardiovascular disease, altered CD163 levels are linked to atherosclerosis and vascular inflammation. These findings underscore the importance of CD163 in both protective and pathological processes.
CD163 antibody is widely applied in flow cytometry, immunohistochemistry, and western blotting. Flow cytometry quantifies macrophage subsets, immunohistochemistry identifies tissue distribution, and western blotting confirms protein size and expression. These applications make CD163 antibody indispensable for immunology and translational research.
By providing validated CD163 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
