| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Amino acids N122-H1039 from the mouse protein were used as the immunogen for the Cd163 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Cd163 Antibody is a anti-CD163 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat. Reported localization: Cell surface, cytoplasmic.
Key elements and design rationale
- Target: CD163
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P, FACS, ELISA
Biological background
Cd163 expression is strongly associated with alternatively activated (M2) macrophages, which are involved in anti-inflammatory processes, tissue repair, and immunosuppression. This makes Cd163 a valuable marker for identifying macrophage polarization states. Employing a Cd163 antibody allows researchers to investigate the dynamics of macrophage activation in response to injury, infection, or disease progression.
Beyond its role in hemoglobin clearance, Cd163 has broader immunomodulatory functions. Engagement of Cd163 can trigger anti-inflammatory signaling cascades and promote the release of heme oxygenase-1, further contributing to cytoprotection. Dysregulated Cd163 expression has been implicated in conditions such as sepsis, atherosclerosis, and chronic inflammatory diseases. In cancer, Cd163-positive macrophages are often linked to tumor-associated macrophages (TAMs), which can support tumor growth and immune evasion. A Cd163 antibody is therefore a powerful tool in both basic immunology and translational research.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
