| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tumor Necrosis Factor Receptor Superfamily Member 8, TNFRSF8 |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human CD30, Fc fusion protein, with C-terminal Avi-Tag, Biotin-Labeled, also known as Tumor Necrosis Factor Receptor Superfamily Member 8, and TNFRSF8, GenBank Accession No. NM_001243.4, a.a. 19-379 expressed in a HEK293 cell expression system. MW = 67 kDa. This protein is fused at the C-terminus to the Fc region of human IgG1 and is enzymatically biotinylated using Avi-tag™ technology. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Product Description
Mammalian (HEK293) ExpressionRecombinant CD30 Human protein is produced using a validated Mammalian (HEK293) expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | P28908 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Amino Acids / Region | 19-379 |
| Molecular Weight | 67 kDa + glycans |
| Formulation | 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol. |
| Storage | At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to … |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
FOR RESEARCH USE ONLY (RUO). Not for diagnostic or therapeutic applications.
This protein was produced in Mammalian (HEK293). Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 19-379. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Useful as a positive control for Western blotting Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Piccinni, M. et al., Journal of Immunology. 1995; 1: 128-133.
- Smith, C.A. et al. Cell. 1993; 73(7): 1349-1360.
