| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, GFP (constitutively expressed), Hygromycin, Puromycin, RFP (constitutively expressed), Zeocin |
| Shipping | |
| Species |
Background
CD32A (FcγRIIa) is a low-affinity Fc gamma receptor expressed on phagocytes such as macrophages and neutrophils. By binding the Fc region of IgG antibodies, CD32A links antibody-coated target cells to effector cells and triggers antibody-dependent cellular phagocytosis (ADCP), a key Fc effector function in which targets are internalized and degraded. The receptor exists as common allotypes that differ in IgG affinity, the higher-affinity H131 and lower-affinity R131 variants, which influence effector responses. ADCP contributes to the therapeutic activity of many monoclonal antibodies, making CD32A and its signaling, often read out through NFAT activation, central to antibody and immuno-oncology research.
Product Description & Applications
The CD32A/NFAT Reporter Lentivirus is an all-in-one immunotherapy reporter system for evaluating the potency of antibody-dependent cellular phagocytosis (ADCP). The construct constitutively expresses the human CD32A high-affinity H131 allotype and carries an NFAT-responsive dual reporter producing secreted Gaussia luciferase together with a fluorescent protein (GFP or RFP). When CD32A engages the Fc region of a target-bound antibody, NFAT is activated and drives reporter expression, providing a quantitative measure of effector-target association in ADCP. It is designed for use with a companion product expressing the low-affinity R131 allotype as a control, and is typically transduced into cell lines such as Jurkat. Supplied as high-titer, VSV-G pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, suitable for difficult-to-transfect primary and thawed cells.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human ADCP Assay under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
