| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CD36 recombinant protein (Position: K233-N439) was used as the immunogen for the CD36 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CD36 Antibody / Platelet glycoprotein 4 is a anti-CD36 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Flow cytometry (FACS), Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: CD36
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): FACS, WB
Biological background
Functionally, CD36 antibody identifies a 472-amino-acid glycoprotein expressed on the plasma membrane of platelets, macrophages, adipocytes, and endothelial cells. CD36 mediates fatty acid uptake and translocation across membranes, contributing to energy homeostasis and lipid storage. It also functions as a pattern recognition receptor in innate immunity, detecting oxidized lipids and apoptotic cells. Engagement of CD36 triggers signaling cascades involving Src-family kinases, MAPKs, and NF-kB, influencing inflammation, oxidative stress, and phagocytosis.
The CD36 gene is located on chromosome 7q21.11 and is expressed in a broad range of tissues, including heart, skeletal muscle, liver, and immune cells. Expression is regulated by transcription factors such as PPARgamma and LXR, which respond to lipid availability and metabolic stress. CD36 plays a central role in coordinating cellular responses to lipid signals and oxidative modification.
Pathologically, CD36 dysregulation contributes to atherosclerosis, insulin resistance, and metabolic syndrome. Overexpression in macrophages promotes foam cell formation and plaque development, while impaired expression leads to altered lipid uptake and cardiac dysfunction. In cancer, CD36 enhances metastatic potential by supporting lipid utilization in tumor cells. Research using CD36 antibody supports studies in lipid biology, cardiovascular disease, and metabolic signaling.
CD36 antibody is validated for western blotting, flow cytometry, and immunohistochemistry to detect cell surface receptors.
Structurally, Platelet glycoprotein 4 consists of two transmembrane domains, a large extracellular glycosylated loop, and short cytoplasmic tails that mediate signal transduction. The receptor interacts with caveolin and other membrane components to form lipid raft-associated complexes. This antibody enables investigation of CD36's function in lipid metabolism, inflammation, and vascular biology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
