| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse Cd63 recombinant protein (Position: A103-I203) was used as the immunogen for the Cd63 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Cd63 Antibody is a anti-CD63 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: CD63
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
CD63, also known as lysosomal-associated membrane protein 3, contains four transmembrane domains and two extracellular loops that mediate interactions with integrins and other tetraspanins. The CD63 antibody enables visualization of this protein on endosomal and plasma membranes, supporting studies of vesicle dynamics and signal transduction. In immune cells, CD63 is upregulated upon activation and translocated to the surface, where it modulates antigen presentation and cell adhesion processes.
In exosome biology, CD63 serves as a canonical marker used to identify and isolate extracellular vesicles derived from endosomal compartments. The CD63 antibody supports flow cytometry, immunoblotting, and immunoelectron microscopy applications to confirm exosome purity and origin. Its detection on vesicular membranes has made it an essential reagent in extracellular vesicle research. CD63 also interacts with proteins such as CD9 and CD81, forming tetraspanin-enriched microdomains that coordinate signaling receptor organization.
Clinically, altered CD63 expression has been associated with cancer progression, immune disorders, and allergic responses. Tumor-derived exosomes enriched in CD63 contribute to intercellular communication that promotes metastasis and immune evasion. The CD63 antibody allows quantitative and localization-based analysis of these vesicles in plasma or tissue samples. In platelets, CD63 acts as a marker of degranulation and activation, supporting research into coagulation and vascular inflammation.
The CD63 antibody performs strongly in western blotting, immunofluorescence, immunohistochemistry, and flow cytometry. It provides distinct membrane and vesicular staining in multiple cell types.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
