| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Conjugate | |
| Host | |
| Immunogen | Unconjugated human brain Thy-1 |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Overview
CD90 FITC is a Mouse monoclonal targeting CD90, supplied as a FITC format for FC workflows. It supports measurement of Human target expression in common experimental systems.
Key elements and design rationale
- Clone: F15-42-1 — consistent clone identity can support panel reproducibility and cross-study comparisons.
- Isotype: IgG1, k — informs selection of matched controls and secondary reagents when relevant.
- Conjugate: FITC — enables direct detection in fluorescence-based assays. Excitation is typically matched to Blue (488nm) lasers in cytometer configurations.
- Host species: Mouse — useful for panel design and control strategy planning.
- Reactivity: Human — interpret staining in the context of species-specific sequence and expression differences.
Key specifications such as clone identity, isotype, and fluorophore conjugation help researchers align panel design, control selection, and instrument configuration with the biological question and sample type.
Biological background
The clone F15-42-1, a mouse monoclonal antibody specifically recognizes a 25-35 kD cell adhesion GPI-anchored protein known as Thy-1 based on its presence on mouse thymocytes or CD90. In humans, CD90 is expressed on stem cells including Mesenchymal Stem Cells (MSCs), Hematopoietic Stem Cells (HSCs) and Keratinocytic Stem Cells (KSCs) and at varying levels on non-lymphoid tissues such as fibroblasts, neurons and activated endothelial cells. CD90 plays important role in the process of inflammation and wound healing by synthesizing and releasing growth factors, cytokines and extracellular matrix components to assist in repairing damaged tissue.
Research relevance and current trends
- High-parameter immunophenotyping: combining CD90 with complementary lineage and activation markers to resolve complex cell states.
- Panel standardization and data comparability: increasing emphasis on consistent reagents, compensation-aware fluorophore choices, and shared gating strategies.
- Integration with single-cell multi-omics: pairing surface marker profiling with transcriptomic or proteomic readouts to connect phenotype to function.
Common research applications
- Flow cytometry: quantify CD90-positive populations and compare expression distributions across conditions or time points.
- Cell sorting: enrich CD90-defined subsets for downstream RNA/protein assays or functional readouts.
Changes in measured signal are typically interpreted in the context of cell subset frequency, activation/differentiation state, and sample processing effects rather than as a standalone readout.
Notes for experimental interpretation
- Fluorophore selection: consider brightness, spectral overlap, and instrument configuration; compensation and spillover can affect apparent population boundaries.
- Biology-driven confounders: activation state, differentiation, and isoform/PTM variation can shift epitope accessibility and apparent expression.
- Control concepts: include matched isotype and fluorescence-minus-one (FMO) controls where appropriate, and interpret results alongside biological positive/negative reference samples.
For antibody-based assays, monoclonal versus polyclonal format can influence epitope recognition breadth and signal consistency. Conjugated antibodies support direct detection and can simplify multicolor panel design when paired with appropriate controls and instrument settings.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
