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Scientific Background
Human CD96, also known as cluster of differentiation 96 and Tactile, GenBank Accession No. NM_198196, a.a. 22-519. This protein is fused at the C-terminus to the Fc region of Human IgG1 and an Avi-tag™, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using AviTag™ technology. MW = 84 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Product Description
Mammalian (HEK293) ExpressionRecombinant CD96 Human protein is produced using a validated Mammalian (HEK293) expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | P40200 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Amino Acids / Region | 22-519 |
| Molecular Weight | 84 kDa |
| Formulation | 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol. |
| Storage | At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to … |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles.
This protein was produced in Mammalian (HEK293). Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 22-519. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Useful as a positive control for Western blot analysis Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Hosen, N. et al., PNAS. 2007; 104(26): 11008-11013.
- Fuchs, A. et al., Journal of Immunology. 2004; 172(7): 3994-3998.
