| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human HRPT2 / CDC73 was used as the immunogen for the CDC73 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CDC73 Antibody / Cell division cycle protein 73 is a anti-CDC73 Rabbit antibody Recombinant Rabbit Monoclonal clone 31C54 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: CDC73
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31C54, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
CDC73 antibody is widely applied in cancer research, molecular biology, and genetic studies. Mutations in CDC73 are associated with hyperparathyroidism-jaw tumor syndrome and parathyroid carcinoma. By detecting CDC73 expression, researchers can investigate its role in tumor suppression and transcriptional control. Loss of CDC73 expression serves as a diagnostic marker for parathyroid malignancy and supports studies of endocrine tumors.
Western blot assays reveal CDC73 protein in nuclear extracts, while immunohistochemistry highlights nuclear localization in normal tissues and its loss in tumor samples. Immunofluorescence provides high-resolution visualization of parafibromin within nuclear transcriptional complexes. These applications make CDC73 antibody valuable for both research and diagnostic contexts.
CDC73 also regulates histone methylation and chromatin remodeling through its interaction with the PAF1 complex. This regulation influences expression of genes involved in cell cycle, apoptosis, and development. By applying CDC73 antibody, scientists can study how transcriptional regulation by parafibromin contributes to normal growth and cancer prevention.
CDC73 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
