| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CDCA8 recombinant protein (Position: D72-K280) was used as the immunogen for the CDCA8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CDCA8 Antibody / Cell division cycle-associated protein 8 / Borealin is a anti-CDCA8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nucleus, Nucleolus.
Key elements and design rationale
- Target: CDCA8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
During mitosis, Borealin localizes to centromeres in metaphase and relocates to the spindle midzone during anaphase, coordinating the transition from chromosome alignment to cytokinesis. Loss of CDCA8 function results in chromosome missegregation, lagging chromatids, and multinucleation, phenotypes commonly associated with aneuploidy and cancer. Expression of CDCA8 peaks during the G2/M phase, reflecting its cell cycle-dependent regulation. Overexpression has been documented in a range of malignancies including breast, lung, and colorectal cancers, correlating with poor prognosis and high proliferative indices.
The CDCA8 antibody is widely used in cell cycle and cancer research to detect Borealin localization and expression dynamics. Immunofluorescence analysis with this antibody reveals distinct centromeric staining during metaphase and midbody localization in late mitosis. Western blotting identifies a 35 kilodalton band corresponding to the full-length protein. Because the chromosomal passenger complex regulates essential mitotic checkpoints, the CDCA8 antibody provides a critical tool for studying mechanisms of mitotic fidelity and tumorigenesis.
CDCA8 cooperates with Aurora B to phosphorylate substrates involved in spindle tension sensing and cytokinesis. Disruption of this interaction impairs CPC activity and leads to mitotic failure. Borealin's ability to bind DNA through its N-terminal domain and oligomerize via its coiled-coil region enables structural stability of the CPC. Inhibition or knockdown of CDCA8 sensitizes tumor cells to microtubule-targeting drugs, highlighting its potential as a therapeutic target. The antibody thus supports translational research exploring CPC inhibitors and mitotic checkpoint modulators in cancer therapy.
In developmental biology, CDCA8 is required for proper cell division during embryogenesis and organogenesis. The CDCA8 antibody facilitates identification of proliferating cells in rapidly dividing tissues and embryonic stem cell cultures.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
