| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse E-cadherin/Cdh1 recombinant protein (Position: Q23-Q708) was used as the immunogen for the Cdh1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Cdh1 Antibody / E-cadherin is a anti-CDH1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: CDH1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, WB
Biological background
Functionally, E-cadherin antibody identifies a 882-amino-acid protein composed of five extracellular cadherin repeats, a transmembrane segment, and a cytoplasmic domain that binds catenins. Through its linkage to beta-catenin and the actin cytoskeleton, E-cadherin forms adherens junctions that regulate tissue morphogenesis and cell migration. Its adhesive strength depends on calcium ions that stabilize the extracellular cadherin domains.
The CDH1 gene is located on chromosome 16q22.1 and is strongly expressed in epithelial tissues, including gastrointestinal tract, liver, mammary gland, and skin. E-cadherin serves as a hallmark of epithelial differentiation, while its downregulation signifies epithelial-to-mesenchymal transition (EMT), a key event in cancer metastasis and wound repair.
Pathologically, loss or mutation of E-cadherin leads to disrupted cell adhesion, increased invasiveness, and hereditary diffuse gastric cancer. CDH1 promoter methylation and transcriptional repression by SNAIL or TWIST contribute to tumor progression. Research using E-cadherin antibody supports studies in cell adhesion, cancer metastasis, and epithelial polarity.
Cdh1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect E-cadherin in tissues and cultured epithelial cells.
Structurally, E-cadherin forms cis and trans dimers that bridge adjacent cell membranes, with its cytoplasmic domain binding to catenins and actin filaments. This antibody facilitates detailed characterization of E-cadherin's role in maintaining epithelial integrity and suppressing invasion.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
