| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CEP55 recombinant protein (Position: E115-K464) was used as the immunogen for the CEP55 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CEP55 Antibody / Centrosomal protein of 55 is a anti-CEP55 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: CEP55
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
CEP55 is encoded by the CEP55 gene on human chromosome 10q23.33. The protein localizes to the centrosome and midbody during late mitosis, coordinating the final stages of cytokinesis. Structurally, CEP55 contains coiled-coil domains and a C-terminal ESCRT- and ALIX-binding region that enables its interaction with ESCRT-I component TSG101 and adaptor ALIX (PDCD6IP). These interactions recruit membrane scission complexes to the midbody, ensuring successful separation of daughter cells following chromosomal segregation.
The CEP55 antibody identifies a 55-60 kilodalton protein in western blot analysis, while immunofluorescence reveals strong midbody and centrosomal staining during telophase and cytokinesis. CEP55 overexpression is frequently observed in tumors, including breast, colon, and lung cancers, where it promotes uncontrolled proliferation and genomic instability. As a consequence, CEP55 is considered both a mitotic marker and a potential therapeutic target in oncology. In normal physiology, it functions as a checkpoint component ensuring that abscission only occurs after proper chromosomal segregation and spindle formation.
Functionally, CEP55 is phosphorylated by PLK1 and Aurora B kinases during mitosis, and these phosphorylation events regulate its recruitment timing to the midbody. Disruption of CEP55 impairs ESCRT recruitment, leading to cytokinesis failure and multinucleated cell formation. Beyond cytokinesis, CEP55 contributes to cilia disassembly and may play a role in signal transduction during cell cycle re-entry.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
