| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Choline Acetyltransferase was used as the immunogen for the CHAT antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CHAT Antibody / Choline Acetyltransferase is a anti-CHAT Rabbit antibody Recombinant Rabbit Monoclonal clone 31C75 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: CHAT
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31C75, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
CHAT antibody is widely used in neuroscience and neurodegenerative disease research. By detecting CHAT, researchers can map cholinergic neuronal populations, study synaptic transmission, and monitor changes in acetylcholine production. Alterations in CHAT activity are linked to disorders including Alzheimer disease, Huntington disease, and amyotrophic lateral sclerosis, where cholinergic dysfunction contributes to pathology.
In western blot assays, CHAT antibody detects protein bands of expected size in brain and spinal cord extracts. Immunohistochemistry highlights cholinergic neurons in the basal forebrain, brainstem, and motor nuclei. Immunofluorescence provides high resolution visualization of CHAT positive axons and synapses, enabling detailed mapping of cholinergic circuitry. ELISA applications support quantitative measurement of CHAT in research samples.
CHAT has been implicated in development and plasticity. During embryogenesis, CHAT expression marks differentiation of cholinergic neurons and is used as a tool to study nervous system maturation. In adult brains, changes in CHAT expression reflect synaptic remodeling and disease related degeneration. By applying CHAT antibody, scientists can study the mechanisms underlying acetylcholine regulation and identify therapeutic strategies for restoring cholinergic signaling.
Beyond neuroscience, CHAT is studied in muscle physiology and autonomic function. It influences neuromuscular transmission and parasympathetic activity, linking it to movement control and visceral regulation. These diverse roles emphasize the value of CHAT antibody across multiple research disciplines.
CHAT antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
