| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human CHP1 was used as the immunogen for the CHP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
CHP1 Antibody / Calcineurin B homologous protein 1 is a anti-CHP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, Perinuclear (Golgi), Cell membrane.
Key elements and design rationale
- Target: CHP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS
Biological background
CHP1 directly binds to the cytoplasmic tails of NHE1, NHE2, and NHE3 isoforms, stabilizing their conformation and enhancing plasma membrane localization. This regulatory role links calcium signaling to intracellular pH regulation and ion balance. CHP1 also associates with calcineurin and other phosphatases, influencing downstream signaling in calcium-dependent pathways. High expression in neurons and cardiac cells underscores its importance in excitable tissues where calcium fluctuations tightly control function and metabolism.
Structurally, CHP1 contains four EF-hand motifs, three of which bind calcium ions, and a myristoylation site that facilitates membrane association. Calcium binding induces conformational changes that alter its interaction with target proteins, providing a molecular mechanism for activity regulation. CHP1 belongs to the calcineurin B homologous protein family, which includes CHP2 and CHP3, all serving as modulators of NHEs and other transporters. CHP1 also co-localizes with NHE1 at the plasma membrane and Golgi apparatus, supporting its dual role in membrane trafficking and ion transport.
Functionally, CHP1 contributes to multiple cellular processes including cytoskeletal organization, vesicle transport, and cell migration. It stabilizes the actin cytoskeleton through NHE1-ERM protein interactions and participates in cellular stress adaptation. CHP1 is implicated in the MAPK and calcium signaling pathways that mediate growth, differentiation, and apoptosis. In neurons, CHP1 interacts with voltage-gated ion channels and regulates excitatory signaling, while in cardiomyocytes it influences contractility by fine-tuning calcium dynamics.
Dysregulation of CHP1 has been linked to neuropathies, cardiac dysfunction, and cancer. Mutations in CHP1 cause autosomal recessive ataxia, characterized by impaired motor coordination and Purkinje cell loss. Abnormal CHP1 expression can also contribute to altered NHE1 activity in glioblastoma and other tumors, affecting intracellular pH and invasive potential. Pathway analysis places CHP1 in ion transport regulation, calcium signaling, and cellular stress adaptation mechanisms.
Immunohistochemical staining using CHP1 antibody shows cytoplasmic and membrane localization, with prominent expression in neurons and cardiac muscle. CHP1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
