| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CHP2 recombinant protein (Position: M1-K196) was used as the immunogen for the CHP2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CHP2 Antibody / Calcineurin B homologous protein 2 is a anti-CHP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, nucleus, cell membrane.
Key elements and design rationale
- Target: CHP2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
CHP2 is expressed predominantly in the heart, skeletal muscle, and certain cancers, including colon and pancreatic tumors. It binds directly to the cytoplasmic tail of sodium-hydrogen exchangers (NHE1, NHE2, NHE3) to stabilize and activate their function. Unlike CHP1, which is ubiquitously expressed, CHP2 expression is more tissue-specific and often upregulated in malignant cells. By regulating NHE activity, CHP2 contributes to pH homeostasis essential for tumor cell survival and motility. Overexpression of CHP2 has been linked to enhanced proliferation and metastatic potential through activation of MAPK and AKT signaling pathways.
The CHP2 antibody is widely used in cell signaling and cancer metabolism research to study calcium-regulated ion transport and tumor biology. Western blotting typically detects a 22 kilodalton band corresponding to the full-length protein, while immunofluorescence shows cytoplasmic and membrane localization consistent with NHE association. Researchers employ the CHP2 antibody to evaluate calcium-dependent signaling events and to compare CHP isoform expression in normal versus transformed cells. Its use extends to studies on cardiac physiology, as CHP2 modulates Ca2+-sensitive processes and contributes to excitation-contraction coupling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
