Cig2 Antibody

Overview

Cig2 is a cyclin that functions as the catalytic subunit of cyclin-dependent kinases (Cdks) in the fission yeast Schizosaccharomyces pombe. It is a B-type, S phase cyclin that is required for both the G1/S and G2/M cell cycle transitions and is expressed in a sharp spike that peaks during the G1/S period. Cig2 binds to Cdc2 (Cdk), and the resulting Cdc2/Cig2 complex controls the G1/S transition of the cell cycle. Disruption of Cig2 delays the onset of mitosis. The expression of the Cig2 gene is dependent on Mlu1-binding factor (MBF), and the protein is destroyed during anaphase by the APC/cyclosome (APC/C) and Skp1/Cullin-1/F-box (SCF), thereby ensuring the spike expression pattern of Cig2. SCF regulates Cig2 levels in a dual manner, transcriptionally and post-translationally, while APC/C only destroys the protein.

This anti-Cig2 antibody is supplied as Purified (Mouse, Monoclonal (mouse origin), clone 3A11/5, Mouse IgG1, kappa, Unconjugated) and is designed to support common target-detection workflows after the on-page specifications.

Key elements and design rationale

• Target: Cig2
• Format: Purified
• Species reactivity: Schizosaccharomyces pombe
• Applications (listed): ELISA, WB
• Conjugate: Unconjugated
• Clone and antibody class: Monoclonal (mouse origin), clone 3A11/5, Mouse IgG1, kappa

Because antibody performance can depend on epitope context, sample preparation, and biological state, interpret signals using appropriate controls and orthogonal evidence when possible.

Biological background

Cig2 is referenced in public gene/protein resources (e.g., UniProt and NCBI Gene), which provide curated names/synonyms, protein features, and pathway context. When designing assays, consider potential isoforms, post-translational modifications, and cell-type specific expression that may influence observed signal.

Research relevance and current trends

• Profiling Cig2 expression across model systems, perturbations, and time points to support mechanistic hypotheses.
• Combining antibody-based detection with multi-omics or imaging readouts to link Cig2 signal with phenotype.
• Using well-matched controls (isotype controls, genetic perturbations, or independent reagents) to strengthen interpretation of target-associated signal.

Common research applications

• ELISA
• WB

Use the listed applications as a starting point and tailor experimental design to your sample type and readout requirements.

Notes for experimental interpretation

• Specificity considerations: closely related family members, isoforms, or PTMs can affect apparent specificity; confirm with independent approaches when critical.
• Controls: include negative controls and, when feasible, genetic or pharmacologic perturbations to support target attribution in your system.
• Species and sample context: differences in sequence, expression, fixation, or extraction conditions can change signal behavior across models.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.