| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Clusterin was used as the immunogen for the Clu antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Clu Antibody / Clusterin is a anti-Clu Rabbit antibody Recombinant Rabbit Monoclonal clone 30C68 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: Clu
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30C68, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, IP
Biological background
Clusterin is expressed in many tissues and exists in secreted and nuclear forms. Research using Clu antibody has shown that secreted Clusterin acts as an extracellular chaperone, binding misfolded proteins and preventing aggregation. This function is particularly important in the brain, where Clusterin helps clear amyloid proteins associated with neurodegenerative disease. Nuclear Clusterin, in contrast, has been linked to pro-apoptotic signaling, underscoring its context-dependent roles.
In neurobiology, Clusterin is associated with Alzheimer's disease and other dementias. Studies with Clu antibody have demonstrated that increased Clusterin levels correlate with amyloid deposition and cognitive decline. Genome-wide association studies have identified CLU variants as risk factors for Alzheimer's disease, further highlighting its importance in neurodegeneration. As a result, Clusterin is being studied as both a biomarker and a therapeutic target.
Clusterin also plays important roles in cancer biology. Research using Clu antibody has revealed that secreted Clusterin can protect tumor cells from stress and apoptosis, promoting therapeutic resistance. Conversely, nuclear Clusterin may enhance apoptosis and suppress tumor growth. These opposing roles reflect the complex regulation of CLU expression and processing in cancer.
Clusterin has additional roles in reproduction, lipid transport, and complement regulation. Studies with Clu antibody have shown that it binds to sperm membranes and influences fertility, while also participating in complement inhibition during inflammation. Its broad functional repertoire makes it a protein of interest across multiple biological disciplines.
Clu antibody is widely used in western blotting, immunohistochemistry, ELISA, and immunofluorescence. Western blotting identifies multiple isoforms, immunohistochemistry reveals expression in brain and tumors, and ELISA quantifies soluble Clusterin in biological fluids. These applications make Clu antibody valuable for translational research.
By providing validated Clu antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
