| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Collagen Type II/COL2A1 recombinant protein (Position: G1217-A1241) was used as the immunogen for the COL2A1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
COL2A1 Antibody / Collagen alpha-1(II) chain is a anti-COL2A1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: COL2A1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Functionally, COL2A1 antibody identifies a 1,484-amino-acid extracellular matrix protein synthesized as a procollagen precursor containing N- and C-terminal propeptides. Following secretion, these propeptides are cleaved to form mature triple-helical type II collagen fibrils composed of three identical alpha-1(II) chains. COL2A1 plays a critical role in chondrocyte differentiation, cartilage development, and matrix maintenance. It also interacts with proteoglycans such as aggrecan to form a hydrated matrix that supports compressive resistance.
The COL2A1 gene is located on chromosome 12q13.11 and is highly expressed in cartilage, eye, and intervertebral disc tissues. Expression is regulated by transcription factors SOX9, RUNX2, and NFATC1, ensuring precise control during skeletal development and repair. COL2A1 is a key marker for chondrogenic lineage commitment in mesenchymal stem cells and serves as a diagnostic biomarker for cartilage integrity.
Pathologically, mutations in COL2A1 cause a spectrum of disorders collectively known as type II collagenopathies, including Stickler syndrome, spondyloepiphyseal dysplasia congenita, and achondrogenesis type II. These conditions are characterized by skeletal malformations, joint defects, and ocular abnormalities. Degradation or reduced expression of COL2A1 contributes to osteoarthritis and intervertebral disc degeneration. Research using COL2A1 antibody supports studies in cartilage biology, skeletal development, and connective tissue disease.
COL2A1 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect extracellular matrix proteins.
Structurally, Collagen alpha-1(II) chain contains a repeating Gly-X-Y motif that forms the characteristic triple-helical structure of fibrillar collagens. The C-terminal propeptide directs trimer assembly, while extensive post-translational modifications including hydroxylation and glycosylation stabilize fibril formation. This antibody facilitates investigation of COL2A1's role in cartilage architecture, skeletal morphogenesis, and connective tissue health.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
