| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human COLGALT1 recombinant protein (Position: A34-D454) was used as the immunogen for the COLGALT1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
COLGALT1 Antibody / Collagen beta(1-O)galactosyltransferase 1 is a anti-COLGALT1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: COLGALT1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
COLGALT1 is encoded by the COLGALT1 gene located on human chromosome 19p13.2. The protein is approximately 739 amino acids long and is a member of the galactosyltransferase family. It forms a functional complex with its homolog COLGALT2 and works sequentially with lysyl hydroxylase (PLOD family) enzymes to glycosylate hydroxylysine residues in collagen alpha chains. This process stabilizes triple helix formation and enhances collagen secretion and cross-linking.
The COLGALT1 antibody detects an 85 kilodalton protein by western blot and shows perinuclear staining under immunofluorescence microscopy consistent with Golgi localization. COLGALT1 activity is required for the synthesis of type I, II, III, and IV collagens. Deficiency or mutation results in defective collagen glycosylation, leading to abnormal extracellular matrix assembly and diseases such as Ehlers-Danlos syndrome and osteogenesis imperfecta-like disorders.
COLGALT1 expression is tightly regulated during development and tissue repair. Increased expression promotes collagen deposition in fibrotic tissues, whereas reduced expression leads to unstable collagen and tissue fragility. It also contributes to tumor stroma remodeling by enhancing extracellular matrix density and rigidity, influencing cancer cell migration and angiogenesis.
As a key enzyme linking collagen biosynthesis and tissue integrity, COLGALT1 serves as a critical target for studying connective tissue disorders and fibrotic diseases.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
