Connexin 43 polyclonal antibody

SKU:BHA11304915
Overview
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Anti-Connexin 43 antibody (Rabbit). Validated for Western Blot, Immunohistochemistry, Immunofluorescence. Reactive against Human, Mouse, Rat targets.
Host Species Rabbit
Species Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Validated Applications Western Blot, Immunohistochemistry, Immunofluorescence
Purification Method Affinity Purification
Options selector
Catalog no. Size
BS6615-50UL 50 uL
BS6615-100UL 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL, 100 uL
  • Lead time: In Stock at Manufacturer options listed in “Availability Content”; other statuses may take longer.
  • Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at −20°C as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No BS6615
Alternative Names GJA1;AVSD3;CMDR;CX43;EKVP;GJAL;HLHS1;HSS;ODDD;PPKCA;EKVP3
Conjugate
  • Unconjugated
Formulation 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Host Rabbit
Immunogen Recombinant fusion protein of human Connexin 43(NP_000156.1).
Molecular Weight ~ 43 kDa
Product Type
  • Primary Antibody
Purity > 95% (by SDS-PAGE)
Reactivity
  • Human
  • Mouse
  • Rat
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product Overview

Research Use Only

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development.

Validated Applications

Western Blot (WB) 500 - 1:2000|IHC
Immunohistochemistry (IHC) 50 - 1:200|IF/ICC
Immunofluorescence (IF/ICC) 50 - 1:200

Key Specifications

Host Species Rabbit
Species Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Purification Affinity Purification
Formulation 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Molecular Weight ~ 43 kDa
Catalog No. BS6615
UniProt P17302
Alternative Names GJA1;AVSD3;CMDR;CX43;EKVP;GJAL;HLHS1;HSS;ODDD;PPKCA;EKVP3

Immunogen / Antigen

Recombinant fusion protein of human Connexin 43(NP_000156.1).

Reactivity Notes

Polyclonal Antibodies

Storage & Handling

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Safety

For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.

What applications has the Connexin 43 polyclonal antibody been validated for?

This antibody has been validated for: Western Blot, Immunohistochemistry, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.

What is the recommended dilution range?

WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Which species does this antibody recognize?

This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.

What is the immunogen / antigen used to generate this antibody?

Recombinant fusion protein of human Connexin 43(NP_000156.1).

How should this antibody be stored and handled?

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.

Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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