| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human CRABP2 was used as the immunogen for the CRABP2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CRABP2 Antibody / Cellular retinoic acid-binding protein 2 is a anti-CRABP2 Rabbit antibody Recombinant Rabbit Monoclonal clone 30C81 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: CRABP2
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30C81, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, FACS
Biological background
CRABP2 antibody is widely applied in developmental biology, cancer research, and dermatology. Retinoic acid is essential for embryonic patterning, limb development, and organogenesis, and CRABP2 ensures delivery of ligand to nuclear receptors. In skin, CRABP2 regulates keratinocyte differentiation and retinoid responses, linking it to dermatological therapies. In cancer biology, CRABP2 expression modulates sensitivity to retinoids, influencing proliferation and apoptosis.
Applications of CRABP2 antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blotting detects CRABP2 protein in cell and tissue lysates, immunohistochemistry maps expression in embryonic and tumor tissues, and immunofluorescence highlights cytoplasmic and nuclear localization. These methods support research into retinoid signaling in diverse contexts.
Dysregulation of CRABP2 is linked to developmental abnormalities, cancer progression, and skin disease. Overexpression promotes retinoid sensitivity in some cancers, while downregulation confers resistance. By applying CRABP2 antibody, researchers can study how retinoid signaling contributes to disease and therapy response.
CRABP2 interacts with nuclear RARs, cytoplasmic enzymes, and signaling molecules. This positions it as a central mediator of vitamin A biology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
