CRCL Antibody / Calcitonin receptor-like receptor

Overview

CRCL Antibody / Calcitonin receptor-like receptor is an antibody targeting CRCL, raised in Rabbit for protein detection and localization studies where these specifications are required.

Key elements and design rationale

• Target: CRCL.
• Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
• Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
• Format: Purified.
• Species reactivity: Human.
• Listed applications: WB, IHC-P (refer to on-page specifications for application-specific guidance).

Biological background

CRCL, also called CALCRL and Calcitonin gene-related peptide type 1 receptor, is a member of the G-protein coupled receptor (GPCR) family, a group of proteins that play a key role in transmitting signals from the outside of the cell to the inside. Specifically, CRCL is known for its involvement in regulating the activity of a neuropeptide called calcitonin gene-related peptide (CGRP), which is involved in pain transmission, migraine development, and vasodilation. CRCL plays a crucial role in mediating the effects of CGRP on various cellular processes. When CGRP binds to CRCL, it triggers a series of signaling cascades that ultimately lead to changes in cell function. For example, activation of CRCL by CGRP can lead to vasodilation in blood vessels, modulation of pain perception, and regulation of inflammatory responses. Dysregulation of this protein has been implicated in various diseases. Abnormalities in CRCL signaling have been linked to migraine development, chronic pain conditions, and cardiovascular diseases.

Research relevance and current trends

• Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
• Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
• Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.

Common research applications

• Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
• Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.

Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.

Notes for experimental interpretation

• Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
• Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
• Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
• Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.