| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human CRCP was used as the immunogen for the CRCP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CRCP Antibody / CGRP receptor component protein is a anti-CRCP Rabbit antibody Recombinant Rabbit Monoclonal clone 30C57 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: CRCP
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30C57, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP
Biological background
CGRP is a neuropeptide involved in vasodilation, pain transmission, and neurogenic inflammation. Functional CGRP receptors require three components: calcitonin receptor like receptor, a receptor activity modifying protein, and CRCP. Research using CRCP antibody has shown that CRCP is required for coupling receptor activation to downstream signaling through G proteins and adenylyl cyclase. Without CRCP, receptor complexes fail to properly signal, underscoring its essential role as a scaffolding factor in peptide receptor function.
CRCP has been extensively studied in the context of migraine. CGRP is a well established mediator of migraine attacks, and antagonists of CGRP receptors have been developed as effective migraine therapies. Studies with CRCP antibody have confirmed that CRCP expression levels influence receptor signaling efficiency, making it an important determinant of migraine susceptibility and treatment response. Because CGRP signaling also regulates vascular tone, CRCP plays a role in cardiovascular homeostasis as well.
Beyond migraine, CRCP contributes to nociception, inflammation, and central nervous system function. Research with CRCP antibody has demonstrated that reduced expression impairs neuronal responses to CGRP, while overexpression amplifies vasodilatory and inflammatory signaling. Its expression in vascular smooth muscle and neuronal tissue highlights its dual roles in vascular biology and sensory processing. Dysregulation of CRCP may therefore contribute to both neurological and cardiovascular disease.
CRCP antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms expression in neuronal and vascular tissues, immunohistochemistry highlights its distribution in smooth muscle and brain, and immunofluorescence demonstrates colocalization with receptor partners. These approaches make CRCP antibody a versatile tool for examining receptor assembly, neuropeptide signaling, and disease mechanisms.
By supplying validated CRCP antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
