| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
CREB (cAMP response element-binding protein) is a basic leucine zipper transcription factor that activates genes containing the cAMP response element (CRE). CREB is a key downstream effector of the cAMP/PKA signaling pathway: rising intracellular cAMP activates protein kinase A, which phosphorylates CREB at serine 133, enabling recruitment of the coactivator CBP/p300 and transcriptional activation. CREB is also regulated by other kinases in response to growth factors and calcium. Through these inputs, CREB controls genes involved in cell proliferation, survival, metabolism, and synaptic plasticity, and its dysregulation is implicated in neurological disorders and cancer, making CRE-driven reporters valuable for studying signal transduction.
Product Description & Applications
The CREB Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of cAMP/PKA pathway activity and CREB-mediated transcription in human or mouse cells. The cAMP response element drives reporter expression in response to cAMP/PKA activation, so signal intensity reflects endogenous pathway activation. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is used to study cAMP/PKA signaling, GPCR responses, CREB-dependent transcription, and pathway modulators. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the cAMP/PKA pathway (CREB-1) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
