| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CREB1 recombinant protein (Position: A7-R270) was used as the immunogen for the CREB1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CREB1 Antibody / cAMP response element-binding protein 1 is a anti-CREB1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Monkey, Mouse, Rat.
Key elements and design rationale
- Target: CREB1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Structurally, CREB1 contains an N-terminal kinase-inducible domain (KID) that undergoes phosphorylation at Ser133 upon activation by protein kinase A (PKA), calcium/calmodulin-dependent protein kinases (CaMKs), or MAPK pathways. This modification enables high-affinity binding to CBP/p300 and transcriptional activation of CRE-dependent genes involved in survival, learning, and glucose homeostasis. The C-terminal basic leucine zipper (bZIP) domain mediates DNA binding and dimerization with other CREB/ATF proteins.
The CREB1 antibody is widely used in signal transduction, neuroscience, and endocrinology research to study gene expression control, synaptic plasticity, and second messenger signaling. Western blot analysis detects a 43 kilodalton band corresponding to CREB1, while immunofluorescence reveals nuclear localization with enrichment at transcriptionally active chromatin. This antibody is also effective for assessing phosphorylation-dependent activation in response to hormones, neurotransmitters, and stress stimuli.
CREB1 activity is crucial for long-term memory formation, neuronal survival, and metabolic regulation. Dysregulation of CREB1 signaling contributes to neurological disorders, depression, diabetes, and tumorigenesis. In cancers, persistent CREB1 activation promotes proliferation and angiogenesis through induction of pro-survival genes such as BCL2 and VEGF. The CREB1 antibody provides a versatile reagent for dissecting transcriptional networks downstream of cAMP and growth factor pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
