| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CREB3 recombinant protein (Position: M1-K352) was used as the immunogen for the CREB3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CREB3 Antibody / Cyclic AMP-responsive element-binding protein 3 is a anti-CREB3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: CREB3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, ELISA
Biological background
Functionally, CREB3 antibody identifies a 371-amino-acid protein anchored in the ER membrane with a cytoplasmic basic leucine zipper (bZIP) domain. Upon ER stress, the N-terminal transcriptional domain of CREB3 is cleaved and translocated to the nucleus, where it activates target genes involved in secretion and Golgi maintenance. CREB3 also regulates acute phase response genes and collagen biosynthesis.
The CREB3 gene is located on chromosome 9p13.3 and is highly expressed in secretory tissues such as liver, pancreas, and intestine. It plays a central role in adapting to increased secretory load and maintaining ER homeostasis under stress conditions.
Pathologically, dysregulation of CREB3 contributes to metabolic disorders, neurodegeneration, and cancer progression by disturbing ER stress response pathways. Overexpression can enhance tumor invasion by modulating extracellular matrix and stress-related signaling genes. Research using CREB3 antibody supports studies in transcriptional regulation, ER stress, and secretory pathway biology.
CREB3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect transcription factors and stress response regulators.
Structurally, Cyclic AMP-responsive element-binding protein 3 contains a bZIP domain for DNA binding and dimerization, along with a luminal stress-sensing region. This antibody enables investigation of CREB3's role in ER stress signaling and transcriptional control.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
