| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human CREB3L2 was used as the immunogen for the CREB3L2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CREB3L2 Antibody / Cyclic AMP-responsive element-binding protein 3-like protein 2 is a anti-CREB3L2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human. Reported localization: ER, Nucleus.
Key elements and design rationale
- Target: CREB3L2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS
Biological background
Functionally, CREB3L2 antibody identifies a 520-amino-acid protein anchored in the ER membrane via a C-terminal transmembrane domain. Upon ER stress or secretory stimulation, the N-terminal cytosolic fragment of CREB3L2 is cleaved and translocates to the nucleus, where it binds CRE-like sequences in target promoters to activate genes related to vesicle trafficking, extracellular matrix organization, and stress tolerance. CREB3L2 acts in concert with other bZIP transcription factors such as ATF6 and CREB3L1 to regulate protein folding and secretion.
The CREB3L2 gene is located on chromosome 7q33 and is highly expressed in secretory tissues such as cartilage, liver, and endocrine glands. It contributes to normal skeletal development and cellular adaptation to increased protein synthesis demands. CREB3L2 is also a critical determinant of collagen biosynthesis during chondrogenesis and wound repair.
Pathologically, translocations involving CREB3L2 and FUS lead to low-grade fibromyxoid sarcoma, resulting in constitutive transcriptional activation and altered differentiation. Aberrant CREB3L2 activity has also been implicated in metabolic and neurodegenerative disorders linked to ER stress. Research using CREB3L2 antibody supports studies in transcriptional control, ER stress, and tumor biology.
CREB3L2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ER-associated transcription factors.
Structurally, Cyclic AMP-responsive element-binding protein 3-like protein 2 features a basic leucine zipper (bZIP) DNA-binding domain, an ER luminal domain involved in sensing stress signals, and a transmembrane region that anchors it to the ER. This antibody aids in studying CREB3L2's role in secretory pathway regulation, collagen production, and tumorigenic gene fusion mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
