| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Adapter molecule crk; Proto-oncogene c-Crk; p38 CRK; CRK; CRKII |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthetic peptide, corresponding to amino acids 200-250 of Human Crk2. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyCrk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway.
Validated Applications
| Immunohistochemistry (IHC) | 1:50~1:200 |
|---|---|
| Immunofluorescence (IF/ICC) | See datasheet for recommended dilution |
Key Specifications
| Host Species | Rabbit |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 42 kDa |
| Catalog No. | BS1079 |
| UniProt | P46108 |
| Alternative Names | Adapter molecule crk; Proto-oncogene c-Crk; p38 CRK; CRK; CRKII |
Immunogen / Antigen
Synthetic peptide, corresponding to amino acids 200-250 of Human Crk2.
Reactivity Notes
Crk2 (P215) polyclonal antibody detects endogenous levels of Crk2 protein.
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Immunohistochemistry, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
IHC: 1:50~1:200 IF: 1:50~1:200
This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Synthetic peptide, corresponding to amino acids 200-250 of Human Crk2.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
