| Field | Specification |
|---|---|
| Alternative Names | NLRP3; C1orf7; CIAS1; NALP3; PYPAF1; NACHT, LRR and PYD domains-containing protein 3; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1CLR1.1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1 |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human NLRP3. AA range:511-560 |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage |
Product Overview
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are s
Polyclonal Rabbit AntibodyValidated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot | Refer to datasheet |
| Immunohistochemistry | Refer to datasheet |
| Immunofluorescence | Refer to datasheet |
| ELISA | Refer to datasheet |
Immunogen & Specificity
Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human NLRP3. AA range:511-560
Subcellular Localization: Cytoplasm, cytosol . Inflammasome . Endoplasmic reticulum . Secreted . Nucleus . In macrophages, under resting conditions, mainly located in the cytosol, on the endoplasmic reticulum. After stimulation with inducers of the NLRP3 inflammasome, mitochondria redistribute in the vicinity of the endoplasmic reticulum in the perinuclear region, which results in colocalization of NLRP3 on the endoplasmic reticulum and PYCARD on mitochondria, allowing the activation of inflammasome assembly. After the induction of pyroptosis, inflammasome specks are released into the extracellular space where they can further promote IL1B processing and where they can be engulfed by macrophages. Phagocytosis induces lysosomal damage and inflammasome activation in the recipient cells (PubMed:24952504). In the Th2 s
UniProt/Swiss-Prot: Q96P20
Product Specifications
| Host Species | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Species Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Observed Band (kDa) | 115kD |
| Storage | -20°C/1 year |
Reactivity Notes
Confirmed reactivity in Human, Mouse, Rat samples; validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA.
Safety & Handling
- For Research Use Only (RUO). Not intended for diagnostic procedures.
- Shipping: cold-chain shipment (typically with ice packs).
- Upon receipt: store at recommended temperature as soon as possible.
- Sales terms and conditions: Please review prior to ordering.
Related Products
Explore additional antibodies and related detection reagents for Cryopyrin in the BioHippo catalog.
This antibody has been validated for Western Blot, Immunohistochemistry, Immunofluorescence. Refer to the product datasheet for recommended dilutions and protocol guidance for each application.
Refer to the datasheet for recommended dilution ranges per application. Optimal dilution may vary by sample type, fixation method, and detection system — a titration experiment is recommended prior to full experimental use.
This antibody has been validated or predicted to recognize Human, Mouse, Rat samples, based on immunogen sequence homology. For unlisted species, contact our team for guidance.
This antibody was raised against a specific peptide or recombinant protein fragment corresponding to Cryopyrin. Exact immunogen details are provided in the product datasheet.
Conjugated formats may be available upon request. Contact the BioHippo team for custom conjugation options including fluorophore, biotin, or enzyme labeling.
BioHippo can support custom antibody formatting and service options for selected products. Contact us to discuss your project requirements.
Potential customization options include:
- Custom conjugation: fluorophore, biotin, enzyme, or nanoparticle labeling available on request for qualified antibodies.
- Bulk or scale-up quantities: volume pricing and custom lead time estimates available for larger orders.
- Alternative formulations: azide-free, carrier protein-free, or lyophilized formats available upon request for selected products.
- OEM and private-label supply: custom packaging and branding available for commercial applications.
Submit your project specifications via the product contact form or reach us at support@ebiohippo.com for a tailored quotation.
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