| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CSDE1/NRU recombinant protein (Position: M1-Q775) was used as the immunogen for the CSDE1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CSDE1 Antibody / Cold shock domain-containing E1 is a anti-CSDE1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: CSDE1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, WB
Biological background
Functionally, CSDE1 antibody recognizes a 794-amino-acid protein containing five cold-shock domains (CSDs) that mediate single-stranded RNA binding. CSDE1 interacts with translation initiation factors and RNA helicases to regulate both cap-dependent and internal ribosome entry site (IRES)-mediated translation. It influences the expression of key regulatory proteins involved in cell growth, apoptosis, and stress adaptation. Through its control of mRNA turnover and translation, CSDE1 coordinates cellular response to nutrient deprivation, hypoxia, and oxidative stress.
The CSDE1 gene is located on chromosome 1p13.2 and is highly expressed in neurons, germ cells, and proliferating tissues. It associates with messenger ribonucleoprotein (mRNP) complexes and localizes to cytoplasmic granules under stress conditions. CSDE1 regulates the translation of c-Fos, Apaf1, and XIAP mRNAs, thereby balancing apoptosis and survival pathways. It also contributes to differentiation processes in embryonic stem cells and neuronal precursors through translational control mechanisms.
In virology, CSDE1 plays a dual role as a host factor that both supports and restricts viral replication, depending on the virus type. For example, it facilitates poliovirus IRES-mediated translation but limits the replication of certain RNA viruses by sequestering viral transcripts. In cancer, dysregulated CSDE1 expression promotes oncogenic translation programs, including MYC and PTEN pathway regulation. Its overexpression correlates with increased metastasis and poor prognosis in melanoma and prostate cancer.
CSDE1 antibody is used for immunoblotting, immunocytochemistry, and RNA immunoprecipitation to study RNA-protein interactions, translation control, and stress response mechanisms. In neuronal models, CSDE1 localization shifts dynamically between cytoplasm and stress granules, reflecting its regulatory versatility. Structural analyses reveal that each CSD contributes distinct RNA-binding affinities, allowing CSDE1 to recognize a broad spectrum of RNA targets and adapt to various signaling environments.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
