| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-terminal-binding protein 2; CtBP2; CTBP2 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthetic peptide, corresponding to amino acids 400-450 of Human CtBP2. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyCtBP1 and the related protein CtBP2 are characterized as C-terminal binding proteins of adenovirus E1A, and they preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation.CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of deltaEF1, a cellular zinc finger-homeodomainprotein, and thereby enhances deltaEF1-induced transcriptional silencing. In addition, CtBP complexes with CtIP, a 125 kDa p
Validated Applications
| Western Blot (WB) | 1:500~1:1000 |
|---|---|
| Immunohistochemistry (IHC) | 1:50~1:200 |
| Immunofluorescence (IF/ICC) | See datasheet for recommended dilution |
Key Specifications
| Host Species | Rabbit |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 48.0 kDa |
| Catalog No. | BS2287 |
| UniProt | P56545 |
| Alternative Names | C-terminal-binding protein 2; CtBP2; CTBP2 |
Immunogen / Antigen
Synthetic peptide, corresponding to amino acids 400-450 of Human CtBP2.
Reactivity Notes
CtBP2 (P432) polyclonal antibody detects endogenous levels of CtBP2 protein.
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunohistochemistry, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Synthetic peptide, corresponding to amino acids 400-450 of Human CtBP2.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
