| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CTRC was used as the immunogen for theCTRC antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CTRC Antibody / Chymotrypsin C is a anti-CTRC Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Rat. Reported localization: Cytoplasm, extracellular.
Key elements and design rationale
- Target: CTRC
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC
Biological background
Structurally, Chymotrypsin C is synthesized as an inactive zymogen (chymotrypsinogen C) and activated by proteolytic cleavage in the small intestine. The mature enzyme is approximately 29 kilodaltons and contains a catalytic triad (His, Asp, Ser) characteristic of serine proteases. CTRC exhibits substrate specificity distinct from other chymotrypsins, favoring cleavage after bulky hydrophobic residues and acting on peptide bonds within pancreatic proenzymes and regulatory proteins.
The CTRC antibody is widely used in digestive biology, enzymology, and pancreatic disease research to study protease regulation, secretion, and zymogen activation. Western blot analysis detects a 29 kilodalton band corresponding to the active enzyme, while immunohistochemistry reveals cytoplasmic staining in pancreatic acinar cells. This antibody supports research on digestive enzyme control, pancreatitis pathogenesis, and exocrine pancreas physiology.
Mutations in CTRC are associated with hereditary chronic pancreatitis and idiopathic pancreatic inflammation, where loss of enzyme function impairs trypsin degradation, leading to excessive intrapancreatic activation. CTRC variants can also affect digestive efficiency and susceptibility to pancreatic stress. Beyond digestion, CTRC influences the proteolytic processing of hormones and growth factors, expanding its role in systemic protease regulation. The CTRC antibody provides a reliable tool for studying enzyme expression, activity regulation, and genetic variants linked to pancreatic disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
