| Field | Specification |
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| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human CXXC5 was used as the immunogen for the CXXC5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CXXC5 Antibody / CXXC-type zinc finger protein 5 is a anti-CXXC5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: CXXC5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS
Biological background
CXXC5 is encoded by the CXXC5 gene located on human chromosome 5q31.2. The protein contains a CXXC-type zinc finger motif enabling DNA interaction, and a Dishevelled (DVL)-binding domain that directly inhibits Wnt signaling by preventing DVL-mediated beta-catenin stabilization. This feedback inhibition is essential for maintaining balanced Wnt activity during embryogenesis and adult tissue homeostasis. CXXC5 also acts as a transcriptional co-regulator by associating with DNA methylation machinery, including TET family enzymes, to maintain epigenetic stability.
Using the CXXC5 antibody, researchers can detect a 34-38 kilodalton protein by western blot and identify nuclear and cytoplasmic localization through immunofluorescence microscopy. CXXC5 expression is found in osteoblasts, endothelial cells, neurons, and fibroblasts, where it coordinates growth factor signaling and differentiation. In bone metabolism, CXXC5 inhibits osteoblast differentiation by suppressing Wnt signaling, while in the vascular system it regulates angiogenic responses. Its dysregulation contributes to conditions such as osteoporosis, impaired wound healing, and tumorigenesis.
Functionally, CXXC5 participates in feedback loops linking Wnt, bone morphogenetic protein, and transforming growth factor beta signaling pathways. It also affects neuronal growth cone development and synaptic plasticity. Recent studies suggest that pharmacologic disruption of the CXXC5-DVL interaction can enhance regenerative responses, including hair follicle regeneration and wound repair.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
