| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cyclomaltodextrin glucanotransferase; EC 2.4.1.19; Cyclodextrin-glycosyltransferase; CGTase |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Bacillus macerans Cyclomaltodextrin glucanotransferase protein (608-713AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This polyclonal anti-Cyclomaltodextrin glucanotransferase antibody raised in Rabbit, tested in ELISA, WB. Designed to detect Cyclomaltodextrin glucanotransferase in Bacillus macerans for others research.
Key elements and design rationale
- Immunogen: Recombinant Bacillus macerans Cyclomaltodextrin glucanotransferase protein (608-713AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
Biological background
Cyclomaltodextrin glucanotransferase (also: Cyclomaltodextrin glucanotransferase, EC 2.4.1.19, Cyclodextrin-glycosyltransferase, CGTase) — studied in Bacillus macerans systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current Cyclomaltodextrin glucanotransferase annotation relevant to others.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
- WB: Confirms MW, distinguishes isoforms under denaturing conditions.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Bacillus macerans; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
Cyclomaltodextrin glucanotransferase is a protein studied in Bacillus macerans biology. This polyclonal antibody raised in Rabbit detects Cyclomaltodextrin glucanotransferase in ELISA, WB applications, IgG isotype.
Reported reactive against Bacillus macerans. Immunogen from Bacillus macerans. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA, WB. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Non-conjugated IgG; requires anti-rabbit-IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin).
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing Cyclomaltodextrin glucanotransferase; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
