| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CYP1B1 recombinant protein (Position: R255-E540) was used as the immunogen for the CYP1B1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CYP1B1 Antibody / Cytochrome P450 1B1 is a anti-CYP1B1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: CYP1B1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, CYP1B1 antibody identifies a 543-amino-acid heme-binding enzyme that catalyzes hydroxylation reactions using NADPH and cytochrome P450 reductase as cofactors. CYP1B1 metabolizes diverse substrates such as estrogens, fatty acids, retinoids, and xenobiotics including polycyclic aromatic hydrocarbons (PAHs). Its activity contributes to detoxification as well as bioactivation of environmental procarcinogens. The enzyme's ability to generate reactive intermediates links it to oxidative DNA damage and tumor initiation.
The CYP1B1 gene is located on chromosome 2p22.2 and contains multiple polymorphisms influencing enzyme activity and disease susceptibility. Mutations in CYP1B1 are a primary cause of primary congenital glaucoma (PCG), due to impaired metabolism of signaling molecules essential for ocular development. In adults, altered CYP1B1 expression is associated with various cancers, including breast, ovarian, and prostate carcinoma, where it modulates estrogen metabolism and tumor progression.
CYP1B1 antibody is used to study xenobiotic metabolism, hormonal regulation, and oxidative stress responses. The enzyme catalyzes 4-hydroxylation of estradiol, producing metabolites that influence estrogen receptor signaling and carcinogenesis. CYP1B1 expression is inducible by environmental toxins such as dioxins and polyaromatic compounds through activation of the aryl hydrocarbon receptor (AhR) pathway. As a result, CYP1B1 serves as a biomarker of chemical exposure and detoxification capacity.
Structurally, CYP1B1 contains a conserved heme-binding domain (Cys-Gly-Gly-His motif) and a hydrophobic membrane anchor. It functions as a monooxygenase by inserting one atom of molecular oxygen into the substrate while reducing the other to water. Regulation occurs via transcriptional control, particularly through AhR-mediated gene induction. Post-translational modifications and redox conditions further modulate its catalytic activity.
A CYP1B1 antibody is suitable for immunoblotting, immunohistochemistry, and enzyme activity assays to investigate tissue-specific expression and regulation. Its detection assists in understanding how xenobiotic metabolism impacts oxidative balance, endocrine signaling, and cancer susceptibility.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
