| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CYP26B1 recombinant protein (Position: N101-A302) was used as the immunogen for the CYP26B1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CYP26B1 Antibody / Cytochrome P450 26B1 is a anti-CYP26B1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER).
Key elements and design rationale
- Target: CYP26B1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
Functionally, CYP26B1 antibody identifies a 512-amino-acid endoplasmic reticulum membrane protein containing a heme-binding domain essential for oxidative metabolism. CYP26B1 converts retinoic acid into hydroxylated metabolites such as 4-hydroxy-retinoic acid and 18-hydroxy-retinoic acid. This reaction ensures proper morphogen gradients required for organ patterning, skeletal formation, and germ cell development.
The CYP26B1 gene is located on chromosome 2p13.2 and is expressed in multiple tissues, including brain, liver, gonads, and embryonic structures. During embryogenesis, CYP26B1 expression establishes regional retinoic acid boundaries, preventing inappropriate activation of retinoic acid-responsive genes. In adults, CYP26B1 regulates tissue regeneration and detoxification of retinoid compounds.
Pathologically, mutations or altered CYP26B1 expression are associated with developmental defects such as craniofacial malformations, skeletal abnormalities, and infertility. Overexpression has also been linked to tumor progression via retinoid resistance. Research using CYP26B1 antibody supports studies in developmental biology, metabolism, and retinoid pharmacology.
CYP26B1 antibody is validated for western blotting, immunohistochemistry, and enzyme assays to detect cytochrome P450 enzymes involved in retinoid metabolism.
Structurally, Cytochrome P450 26B1 features conserved P450 motifs, including the heme-binding cysteine loop and oxygen activation domains. This antibody aids studies on CYP26B1's role in retinoid turnover, tissue patterning, and disease development.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
