| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human CYP2W1 was used as the immunogen for the CYP2W1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CYP2W1 Antibody / Cytochrome P450 2W1 is a anti-CYP2W1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human. Reported localization: ER, cell membrane.
Key elements and design rationale
- Target: CYP2W1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC
Biological background
Functionally, CYP2W1 antibody identifies a 490-amino-acid membrane-bound enzyme localized to the endoplasmic reticulum. CYP2W1 utilizes NADPH-cytochrome P450 reductase as an electron donor to catalyze monooxygenation of various substrates including fatty acids, indole derivatives, and xenobiotics. Although minimally expressed in adult tissues, CYP2W1 is highly active in embryonic development and selectively re-expressed in certain cancers, where it contributes to prodrug metabolism and tumor-specific bioactivation.
The CYP2W1 gene is located on chromosome 7q22.3 and is expressed predominantly in colon, adrenal gland, and fetal tissues. Expression is regulated by epigenetic mechanisms such as DNA methylation and histone modification, restricting CYP2W1 activity to specific developmental and pathological contexts.
Pathologically, CYP2W1 has gained attention as a tumor-specific biomarker and therapeutic target due to its selective expression in colon, adrenal, and lung cancers. It catalyzes activation of several anticancer prodrugs, providing potential for targeted therapy. Conversely, its aberrant activity may contribute to tumor progression by metabolizing endogenous substrates into bioactive lipids. Research using CYP2W1 antibody supports studies in pharmacology, oncology, and drug metabolism.
CYP2W1 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect cytochrome P450 enzymes.
Structurally, Cytochrome P450 2W1 contains a conserved P450 fold with heme-binding motifs (FGxGPR and Cys-pocket) and a hydrophobic N-terminal region for membrane anchoring. The active site accommodates small aromatic and aliphatic compounds, enabling broad substrate specificity. This antibody allows investigation of CYP2W1's function in tissue-specific metabolism and its emerging role in cancer therapeutics.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
