| Field | Specification |
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| Host | |
| Immunogen | E.coli-derived human CYP7B1 recombinant protein (Position: Q127-D391) was used as the immunogen for the CYP7B1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CYP7B1 Antibody / 25-hydroxycholesterol 7-alpha-hydroxylase is a anti-CYP7B1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER).
Key elements and design rationale
- Target: CYP7B1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Localized to the endoplasmic reticulum membrane, CYP7B1 uses NADPH-cytochrome P450 reductase as an electron donor for hydroxylation reactions. It is highly expressed in liver, brain, and steroidogenic tissues, where it contributes to both cholesterol catabolism and neuroactive steroid metabolism. By regulating oxysterol levels, CYP7B1 influences liver lipid homeostasis and protects against cholesterol accumulation and neurotoxicity.
The CYP7B1 antibody is widely used in metabolism, neurobiology, and endocrinology research to study bile acid biosynthesis, cholesterol turnover, and steroid regulation. Western blot analysis detects a 55 kilodalton band corresponding to CYP7B1, while immunofluorescence reveals endoplasmic reticulum localization in hepatocytes and neurons. This antibody provides a means to assess CYP7B1 expression under physiological and pathological conditions, including liver disease and metabolic disorders.
Mutations in CYP7B1 cause hereditary spastic paraplegia type 5A and neonatal cholestasis, demonstrating the enzyme's essential role in cholesterol detoxification and neuronal maintenance. Altered CYP7B1 expression has also been linked to atherosclerosis, Alzheimer's disease, and hormonal dysregulation. The CYP7B1 antibody supports research into cholesterol metabolism, neurosteroid synthesis, and disease pathophysiology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
