| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived Danre Lmo2 recombinant protein (amino acids M1-V159) was used as the immunogen for the Danre Lmo2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Danre Lmo2 Antibody / Rhombotin 2 is a anti-LMO2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Nucleus, Cytoplasm, Cell surface.
Key elements and design rationale
- Target: LMO2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Lmo2 does not directly bind to DNA but functions as a scaffold protein, forming complexes with other transcription factors to regulate gene expression. During zebrafish embryogenesis, Lmo2 is expressed in the intermediate cell mass, the site of primitive blood cell formation, and in the developing vasculature. It is required for the emergence of both erythroid and myeloid lineages, as well as for the proper development of endothelial cells.
Loss of Lmo2 function in zebrafish results in severe defects in blood cell formation and vascular patterning, highlighting its central role in mesodermal differentiation. Because of its strong evolutionary conservation and defined expression patterns, zebrafish Lmo2 serves as a powerful model for studying hematopoiesis, angiogenesis, and transcriptional networks involved in early development.
Lmo2 is also relevant to human health, as misregulation of Lmo2 has been associated with leukemia. This makes zebrafish Lmo2 a valuable tool in both basic research and disease modeling related to blood development and cancer biology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
