| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human DAZAP1 was used as the immunogen for the DAZAP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DAZAP1 Antibody / DAZ associated protein 1 is a anti-DAZAP1 Rabbit antibody Recombinant Rabbit Monoclonal clone 29D87 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: DAZAP1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29D87, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
DAZ associated protein 1 contains RNA recognition motifs that allow it to bind specific transcripts and influence splicing. Research using DAZAP1 antibody has demonstrated that it regulates pre-mRNA splicing of genes required for germ cell maturation. Its role in testis development makes it critical for fertility, and disruption of DAZAP1 function can impair spermatogenesis. Beyond reproduction, DAZAP1 is expressed in somatic tissues where it contributes to general RNA regulation.
DAZAP1 interacts with a variety of splicing factors and RNA processing proteins. Studies with DAZAP1 antibody have shown that it modulates exon inclusion or exclusion in target transcripts, thereby shaping tissue-specific gene expression. Alterations in DAZAP1 expression influence cell differentiation and development, suggesting a broader biological impact beyond germline function.
In disease contexts, DAZAP1 has been linked to cancer and developmental abnormalities. Research with DAZAP1 antibody has revealed that altered expression affects cell cycle regulation and apoptosis. Its misregulation in tumors suggests a potential role in oncogenesis, possibly through changes in RNA splicing that promote malignant phenotypes. These findings highlight DAZAP1 as both a fertility factor and a regulator of cell fate.
DAZAP1 antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein size and isoforms, immunohistochemistry demonstrates expression in testis and other tissues, and immunofluorescence shows nuclear localization with splicing speckles. These methods make DAZAP1 antibody versatile in RNA biology research.
By providing validated DAZAP1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
