| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human DCAF13 was used as the immunogen for the DCAF13 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DCAF13 Antibody / DDB1- and CUL4-associated factor 13 is a anti-DCAF13 Rabbit antibody Recombinant Rabbit Monoclonal clone 32D85 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: DCAF13
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32D85, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, IP
Biological background
DDB1- and CUL4-associated factor 13 belongs to the family of WD repeat proteins that serve as adaptors for CUL4-based E3 ligases. Research using DCAF13 antibody has shown that it recognizes substrates and recruits them to the ligase complex, ensuring precise turnover of proteins that regulate transcription and cell cycle progression. This function is critical for maintaining protein homeostasis and genomic stability.
Studies with DCAF13 antibody have revealed that it plays roles in embryonic development and tumorigenesis. In embryonic cells, DCAF13 supports proliferation by targeting key regulators of transcription and signaling for degradation. In cancers, overexpression of DCAF13 enhances tumor growth and survival by destabilizing tumor suppressor proteins. These observations highlight the importance of DDB1- and CUL4-associated factor 13 in growth control and oncogenesis.
DCAF13 has also been implicated in stress responses. Research using DCAF13 antibody has demonstrated that the protein participates in DNA damage repair pathways, regulating protein stability during genotoxic stress. By coordinating protein turnover with DNA repair, DCAF13 contributes to genome integrity.
DCAF13 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects endogenous protein levels, immunohistochemistry reveals expression in proliferative tissues, and immunofluorescence highlights nuclear localization consistent with transcriptional regulation. These approaches make DCAF13 antibody valuable for investigating ubiquitin signaling and cancer biology.
By supplying validated DCAF13 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
