| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human XTP3TPA/DCTPP1 recombinant protein (Position: R22-T170) was used as the immunogen for the DCTPP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DCTPP1 Antibody / dCTP pyrophosphatase 1 / XTP3TPA is a anti-DCTPP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse. Reported localization: Cytoplasm, Nucleus.
Key elements and design rationale
- Target: DCTPP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IHC, WB
Biological background
Located primarily in the nucleus and mitochondria, DCTPP1 acts as a homotetrameric hydrolase with substrate specificity for dCTP and 5-methyl-dCTP. By regulating the deoxynucleotide pool, it prevents mispairing and epigenetic instability caused by cytosine modifications. Its activity supports accurate DNA replication and limits the formation of base substitution mutations. DCTPP1 expression increases under oxidative stress and in proliferating cells to safeguard replication accuracy.
The DCTPP1 antibody is widely used in DNA metabolism, cancer, and oxidative stress research to investigate nucleotide sanitization, genome maintenance, and replication control. Western blot analysis detects a 21 kilodalton band corresponding to DCTPP1, while immunofluorescence reveals both nuclear and mitochondrial localization depending on cell type. This antibody enables researchers to explore DNA repair dynamics and nucleotide pool balance in diverse biological systems.
Loss or dysregulation of DCTPP1 is associated with increased mutation rates, mitochondrial dysfunction, and genomic instability. Elevated expression has been observed in rapidly dividing tumor cells, where it supports proliferation by maintaining balanced nucleotide pools. The DCTPP1 antibody provides a powerful tool for studying DNA replication accuracy, oxidative defense, and cancer cell metabolism.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
