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| Immunogen | A synthesized peptide derived from human MLD was used as the immunogen for the DEGS1 antibody. |
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Overview
DEGS1 Antibody / DES1 / Dihydroceramide desaturase-1 is a anti-DEGS1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30D03 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: DEGS1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30D03, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Dihydroceramide desaturase 1 resides in the endoplasmic reticulum and is part of a larger network of enzymes controlling sphingolipid levels. By converting dihydroceramide to ceramide, DEGS1 regulates the balance between precursors and bioactive sphingolipids. Research with DEGS1 antibody has shown that altered activity of this enzyme shifts cellular lipid composition, leading to changes in apoptosis sensitivity and membrane structure. Ceramides produced by DEGS1 also participate in signaling pathways that control stress responses, mitochondrial function, and immune activity.
Mutations in DEGS1 cause rare hereditary spastic paraplegia and leukodystrophy, highlighting the enzyme's role in nervous system development and maintenance. Patients with DEGS1 mutations show impaired myelination, progressive neurodegeneration, and developmental delay. Studies using DEGS1 antibody have demonstrated that defective enzyme activity leads to accumulation of dihydroceramides and reduced ceramide production, disrupting neuronal signaling and survival. These findings underscore the enzyme's essential role in neurobiology and its potential as a therapeutic target.
In cancer, altered sphingolipid metabolism has been linked to tumor progression and treatment resistance. DEGS1 contributes to the regulation of apoptosis sensitivity, and reduced ceramide levels can enable survival of malignant cells under stress. Research with DEGS1 antibody has associated aberrant expression with poor prognosis in certain cancers, making it a potential biomarker and therapeutic target. Beyond oncology, sphingolipid imbalance has been implicated in metabolic diseases such as diabetes and obesity, further expanding the relevance of DEGS1.
DEGS1 antibody is applied in western blotting, immunohistochemistry, and lipidomic studies. Western blotting confirms protein expression levels, while immunohistochemistry highlights tissue specific distribution in brain, liver, and metabolic organs. Functional assays using DEGS1 antibody in combination with lipidomics allow researchers to connect enzyme expression with lipid composition changes, providing a systems level view of sphingolipid biology.
By supplying validated DEGS1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
