| Field | Specification |
|---|---|
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Deoxyribonuclease I (DNase I) is a nuclease found in a wide variety of cells and tissues. It targets and cleaves the phosphodiester bond adjacent to the pyrimidine to produce polynucleotides with a phosphate group at the 5' end and a hydroxyl group at the 3' end. The average digestion product is about the size of a polynucleotide. DNase can catalyse different forms of DNA such as single-stranded DNA, double-stranded DNA and even chromatin (its cutting rate is affected by histones). The optimum operating range is pH 7-8. The activity of DNase is Ca2+ dependent and can be activated by divalent metal ions such as Co2+, Mn2+ and Zn2+. 5 mM Ca2+ can protect the enzyme from hydrolysis. In the presence of Mg2+, the enzyme can randomly recognise and cut any site on either DNA chain; in the presence of Mn2+, it can recognise both DNA chains simultaneously and cut at almost the same site. DNase I was first isolated from pancreas, and mammalian pancreas is still a major source of the enzyme.
This product is derived from bovine pancreas and consists of four chromatographically distinct components A, B, C and D in a molar ratio of 4:1:1, with component D being very small. It is often used in molecular biology experiments to remove DNA from proteins or to create gaps in DNA to allow the insertion of labelled bases into DNA. This product is supplied as a lyophilised powder containing calcium chloride with an enzyme activity of ≥2000 Kunitz units/mg protein.
Features
High activity
Low residual RNase
Stable Quality: No variations between batches
Applications
DNA removal in RNA extraction; DNA removal in in vitro transcription; DNase I blotting; nick translation; RNA purification;
Specification
|
CAS NO. |
9003-98-9 |
|
Molecular Weight |
~31 kDa |
|
Kunitz Units |
≥2000 Kunitz Units/mg protein |
|
Type |
Type IV |
|
7～8 |
|
|
Appearance |
White to light brown freeze-dried powder |
|
Purity |
Protein: ≥80% by Biuret |
|
Solubility |
0.15 M NaCl：5 mg/mL, colorless and transparent solution |
|
Activators |
Various Glycerol Content: Contains Glycerol |
Store this enzyme at Room Temperature and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Ambient and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
DNA removal in RNA extraction; DNA removal in in vitro transcription; DNase I blotting; nick translation; RNA purification;. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that degrades 1 µg of DNA or RNA substrate to acid-soluble form in 30 min at 37°C under defined buffer conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Nuclease activity typically requires divalent metal ion cofactors (Mg²⁺ or Mn²⁺ at 1–5 mM). Activity is inhibited by chelating agents such as EDTA (≥1 mM), high salt concentrations, and reducing agents such as DTT at elevated concentrations. Heat inactivation (65–75°C for 15 min) is effective for most nucleases, allowing removal of enzyme activity after digestion without column purification.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
- Xu Y, Hu Y, Xu T, et al. RNF8-mediated regulation of Akt promotes lung cancer cell survival and resistance to DNA damage. Cell Rep. 2021;37(3):109854. doi:10.1016/j.celrep.2021.109854(IF:9.423)
- Jain S, Hu C, Kluza J, et al. Metabolic targeting of cancer by a ubiquinone uncompetitive inhibitor of mitochondrial complex I. Cell Chem Biol. 2022;29(3):436-450.e15. doi:10.1016/j.chembiol.2021.11.002(IF:8.116)
- Sun H, Chen D, Zhan S, et al. Design and Discovery of Natural Cyclopeptide Skeleton Based Programmed Death Ligand 1 Inhibitor as Immune Modulator for Cancer Therapy. J Med Chem. 2020;63(19):11286-11301. doi:10.1021/acs.jmedchem.0c01262(IF:6.205)
- Zhao X, Hu S, Zeng L, et al. Irradiation combined with PD-L1-/- and autophagy inhibition enhances the antitumor effect of lung cancer via cGAS-STING-mediated T cell activation. iScience. 2022;25(8):104690. Published 2022 Jun 30. doi:10.1016/j.isci.2022.104690(IF:6.107)
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