| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Deoxyribonuclease I (DNase I), also known as Deoxyribonuclease I, is a nuclease found in various cells and tissues, classified as an endonuclease that targets the phosphodiester bonds adjacent to pyrimidines, producing polynucleotides with a phosphate group at the 5' end and a hydroxyl group at the 3' end, with an average digestion product minimum of a tetranucleotide. DNase can catalyze various forms of DNA, such as single-stranded DNA, double-stranded DNA, and even chromatin (the cleavage rate is influenced by histones). The optimal working range is pH 7-8, and the activity of DNase depends on Ca 2+, and can be activated by divalent metal ions such as Co 2+, Mn 2+, Zn 2+, etc. 5 mM Ca 2+ can protect the enzyme from hydrolysis. In the presence of Mg 2+, the enzyme can randomly recognize and cleave at any site on either strand of DNA; Whereas under the conditions of Mn 2+, it can simultaneously recognize both strands of DNA and cleave at nearly the same site. DNase I was first isolated from the pancreas, and to this day, the pancreas of mammals remains one of the main sources of this enzyme.
This product is derived from bovine pancreas and is commonly used in molecular biology experiments to remove DNA from proteins or to introduce nicks into DNA for the insertion of labeled bases. This product is supplied in powder form, with enzyme activity ≥ 2000 Kunitz Units/mg protein.
Features
Lyophilized powder
Low RNase residue
Applications
Used for bioprocesses of removing DNA from protein and cells samples
Single-cell suspension preparation to decreases viscosity by removing DNA
Radioactive labelling and DNA nick translation
DNase footprinting
Eliminating genomic DNA from RNA preparations
Specifications
|
CAS Number |
9003-98-9 |
|
MW |
~31 kDa |
|
Kunitz Units |
≥2000Kunitz Units/mg protein |
|
Type |
Type IV |
|
Optimal pH |
7～8 |
|
Appearance |
White to light yellow powder |
|
Purity |
Protein: ≥80% by Biuret |
|
Activators |
Various divalent metal ions such Glycerol Content: Contains Glycerol |
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Used for b ioprocess es of remov ing DNA from protein and cells samples Single-cell suspension preparation to decreases viscosity by removing DNA Radioactive labelling and DNA nick translation DNase footprinting Eliminating genomic DNA from RNA preparations. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that degrades 1 µg of DNA or RNA substrate to acid-soluble form in 30 min at 37°C under defined buffer conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Nuclease activity typically requires divalent metal ion cofactors (Mg²⁺ or Mn²⁺ at 1–5 mM). Activity is inhibited by chelating agents such as EDTA (≥1 mM), high salt concentrations, and reducing agents such as DTT at elevated concentrations. Heat inactivation (65–75°C for 15 min) is effective for most nucleases, allowing removal of enzyme activity after digestion without column purification.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
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- Huang Y, Chen Y, Zhou S, et al. Dual-mechanism based CTLs infiltration enhancement initiated by Nano-sapper potentiates immunotherapy against immune-excluded tumors. Nat Commun. 2020;11(1):622. Published 2020 Jan 30. doi:10.1038/s41467-020-14425-7(IF:12.121)
- Wang Y, Gong X, Li J, et al. M2 macrophage microvesicle-inspired nanovehicles improve accessibility to cancer cells and cancer stem cells in tumors. J Nanobiotechnology. 2021;19(1):397. Published 2021 Nov 27. doi:10.1186/s12951-021-01143-5(IF:10.435)
- Liu J, Shen Z, Tang J, et al. Extracellular DNA released by glycine-auxotrophic Staphylococcus epidermidis small colony variant facilitates catheter-related infections. Commun Biol. 2021;4(1):904. Published 2021 Jul 22. doi:10.1038/s42003-021-02423-4(IF:6.268)
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DNase I Applications
