DFFB Antibody, Biotin conjugated

Overview

This is a polyclonal anti-DFFB antibody raised in Rabbit, conjugated with Biotin, with confirmed utility in ELISA. It is designed to detect DFFB protein in Human and supports researchers working in cell biology contexts.

Key elements and design rationale

• Immunogen: Recombinant Human DNA fragmentation factor subunit beta protein (108-219AA ) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
• Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
• Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
• Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
• Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
• Biotin conjugate: Amplified detection via streptavidin/avidin reporters. Block endogenous biotin in biotin-rich tissue samples.

Biological background

DFFB (also referred to as CAD, Caspase activated deoxyribonuclease, Caspase activated DNase, Caspase activated nuclease, Caspase-activated deoxyribonuclease, Caspase-activated DNase, Caspase-activated nuclease, CPAN, Deoxyribonuclease III, caspase activated, DFF 40, DFF-40, DFF2, DFF40, Dffb, DFFB_HUMAN, Didff, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta, DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase), DNA fragmentation factor, 40 kD beta subunit, DNA fragmentation factor, 40 kD, beta polypeptide, DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase), OTTHUMP00000003633) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of DFFB biology in cell biology.

Common research applications

• ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.

Notes for experimental interpretation

• Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
• Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
• Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
• Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.

This product is a polyclonal antibody purified from rabbit antiserum.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.