| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LGP2/DHX58 recombinant protein (Position: A38-S658) was used as the immunogen for the DHX58 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DHX58 Antibody / DExH-box helicase 58 is a anti-DHX58 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: DHX58
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
DHX58 is encoded by the DHX58 gene located on human chromosome 17q21.1. The protein is approximately 678 amino acids long and contains a helicase core domain, ATP-binding motifs, and a C-terminal regulatory domain. Unlike RIG-I and MDA5, DHX58 lacks the N-terminal CARD domains required for direct signaling, functioning instead as a modulator of their activity. It localizes to the cytoplasm and associates with stress granules and viral replication complexes during infection.
The DHX58 antibody detects a 75 kilodalton band by western blot and exhibits punctate cytoplasmic staining by immunofluorescence microscopy. DHX58 acts as a positive or negative regulator of antiviral signaling depending on context. It binds double-stranded RNA and modulates the activity of MDA5 and RIG-I, fine-tuning the magnitude and duration of type I interferon responses. During viral infection, DHX58 helps discriminate self from non-self RNA, preventing inappropriate immune activation.
Mutations or altered expression of DHX58 affect susceptibility to viral pathogens such as influenza, hepatitis C, and coronaviruses. It also influences autoimmunity, where excessive activation of the RLR pathway leads to chronic inflammation and tissue damage. Beyond immunity, DHX58 participates in RNA metabolism and stress granule dynamics, linking it to translational control during cellular stress.
As a key regulator of antiviral sensing, DHX58 provides a molecular handle for understanding innate immune balance and pathogen recognition.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
