| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human DIS3 recombinant protein (Position: H43-K878) was used as the immunogen for the DIS3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DIS3 Antibody / Exosome complex exonuclease RRP44 is a anti-DIS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: DIS3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, ICC, WB
Biological background
Functionally, DIS3 antibody identifies a 958-amino-acid cytoplasmic and nuclear protein that associates with the multi-protein RNA exosome complex. DIS3 degrades defective, misprocessed, or surplus RNA species, including rRNA, snoRNA, snRNA, and mRNA. It possesses two catalytic domains�an RNB domain for exonucleolytic degradation and a PIN domain for endonucleolytic cleavage�allowing versatile RNA processing under various physiological conditions. By maintaining RNA homeostasis, DIS3 contributes to normal gene expression and cellular viability.
The DIS3 gene is located on chromosome 13q22.1 and is expressed ubiquitously in proliferative tissues. Its activity is regulated through complex assembly, post-translational modification, and subcellular localization. DIS3 operates in both the nucleus, where it processes pre-rRNA and small noncoding RNAs, and the cytoplasm, where it degrades aberrant mRNA species.
Pathologically, mutations in DIS3 cause multiple myeloma, acute myeloid leukemia, and related hematologic malignancies. These mutations impair RNA degradation, leading to accumulation of defective transcripts and deregulated gene expression. Loss of DIS3 function disrupts RNA quality control and contributes to oncogenic transformation. Research using DIS3 antibody supports studies in RNA metabolism, gene regulation, and cancer genomics.
DIS3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect exosome complex components.
Structurally, Exosome complex exonuclease RRP44 contains an N-terminal PIN domain for endonucleolytic activity and a central RNB catalytic core typical of RNase II family enzymes. The C-terminal S1 and CSD domains enable RNA substrate recognition and binding. This antibody aids in investigating DIS3's role in RNA exosome-mediated decay and RNA quality control in normal and malignant cells.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
