| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human DKC1 recombinant protein (Position: R19-R447) was used as the immunogen for the DKC1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DKC1 Antibody / Dyskerin is a anti-DKC1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus, Nucleolus, Cytoplasm.
Key elements and design rationale
- Target: DKC1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
Functionally, DKC1 antibody identifies a 514-amino-acid protein localized in the nucleolus and Cajal bodies. DKC1 associates with NOP10, NHP2, and GAR1 to form the H/ACA ribonucleoprotein complex that modifies rRNA and snRNA. It also binds the telomerase RNA component (TERC), stabilizing the telomerase complex for telomere elongation.
The DKC1 gene is located on chromosome Xq28 and is ubiquitously expressed in dividing and metabolically active cells. Dyskerin function is vital for maintaining ribosome synthesis, RNA quality control, and telomere homeostasis. Its activity directly influences cell growth and chromosomal stability.
Pathologically, mutations in DKC1 cause X-linked dyskeratosis congenita, a rare disorder characterized by bone marrow failure, premature aging, and cancer predisposition. Reduced DKC1 function leads to defective rRNA modification, impaired telomerase activity, and genomic instability. Research using DKC1 antibody supports studies in RNA processing, telomere biology, and genetic diseases.
DKC1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect pseudouridine synthases and nucleolar proteins.
Structurally, Dyskerin features a TruB-like catalytic domain with conserved aspartate essential for pseudouridylation activity, and a nuclear localization signal directing nucleolar import. This antibody aids in elucidating DKC1's role in ribonucleoprotein assembly and RNA modification fidelity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
