| Field | Specification |
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| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human DMPK was used as the immunogen for the DMPK antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DMPK Antibody / Myotonin-protein kinase is a anti-DMPK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: DMPK
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
Myotonin-protein kinase localizes to the sarcolemma, cytoplasm, and nuclear envelope of muscle cells, where it phosphorylates substrates involved in cytoskeletal organization and ion channel regulation. It contributes to the maintenance of sarcomere integrity and calcium signaling required for normal muscle contraction. The DMPK antibody is used to assess protein expression and localization, providing insights into how loss or mutation of this kinase disrupts muscular homeostasis.
Expansion of CTG trinucleotide repeats in the 3? untranslated region of the DMPK gene causes DM1, a multisystem disorder characterized by muscle weakness, myotonia, and cardiac conduction defects. This repeat expansion leads to toxic RNA accumulation, altered splicing of multiple genes, and reduced DMPK protein levels. The DMPK antibody supports investigations into these molecular effects by allowing direct measurement of protein expression in affected tissues. Studies using this reagent have clarified that decreased Myotonin-protein kinase activity contributes to muscle atrophy and electrical instability in DM1 patients.
Beyond myotonic dystrophy, DMPK signaling intersects with Rho GTPase and MAPK pathways, suggesting roles in cytoskeletal dynamics and stress responses. The DMPK antibody is a valuable reagent for studying these signaling networks and their involvement in cardiac hypertrophy, smooth muscle contractility, and cell migration. In cardiac research, immunohistochemical detection using the DMPK antibody reveals its presence in intercalated discs and perinuclear regions, correlating with its function in maintaining contractile coordination.
Experimental applications for the DMPK antibody include western blotting, immunofluorescence, and immunohistochemistry. These assays permit detailed characterization of Myotonin-protein kinase in tissue and cellular models.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
